PMC

Purification and characterization of the minor (Mfa1) fimbria. (A) Elution profile of the 67-kDa fimbria on DEAE-Sepharose CL-6B, showing a peak that eluted with 0.3 M NaCl. (B) SDS-PAGE analysis of the minor fimbria. Lane 1, MW standard; lane 2, Coomassie blue stain showing the 67-kDa minor fimbria; lane 3, silver stain showing the single band of the minor fimbria (arrow). (C) Transmission electron micrograph of the purified minor fimbria showing 100- to 200-nm fibers. Bar, 100 nm.

D₂ receptor blockade restores the effects of mPFC inactivation on the vSub–NAc pathway. A, Peristimulus time histogram (5 min samples; 150 sweeps) of a typical response to fimbria stimulation recorded from a NAc neuron 5 min after intravenous sulpiride (5 mg/kg) injection and 20 and 40 min after tetanic stimulation of hippocampal afferents. The arrows indicate the time at which the fimbria is stimulated. B, LTP is induced 10 min after tetanic stimulation of the fimbria and lasts for >35 min (n = 7). C, Peristimulus time histogram (5 min samples) of a typical response to fimbria stimulation recorded from a NAc neuron 5 min after intravenous injection of sulpiride, 20 min after fimbria tetanus, and 20 min after TTX infusion into the mPFC. The arrows indicate the time at which the fimbria is stimulated (n = 9). D, LTP is induced 10 min after fimbria tetanic stimulation. Ten minutes after infusion of TTX into the mPFC, there is a return to baseline spike probability. E, Peristimulus time histogram (5 min samples) of a typical response to fimbria stimulation recorded from a NAc neuron, 10 min after fimbria tetanus, 5 min after intravenous injection of sulpiride, and 10 min after TTX infusion into the mPFC. The arrows indicate the time at which the fimbria is stimulated. F, LTP is induced 15 min after fimbria tetanic stimulation. When intravenous injection of sulpiride is performed after tetanus, no return to baseline spike probability is observed after infusion of TTX in the mPFC (n = 5). Error bars indicate SEM. *p < 0.05.

Gene expression analysis identifying differences in gene ontology pathway representation and gene upregulation and downregulation between fimbria and ampulla. (A) Heat map of supervised hierarchical clustering revealed significant differences (2-fold change, p < 0.05) in gene expression between ampulla and fimbria. As seen previously, some follicular and luteal phase genes segregate by fimbria and ampulla of the normal FTE expression pattern. (B) Volcano plots showing a comparison of i: fimbria compared to ampulla, independent of ovulatory phase, ii: fimbria compared to ampulla in the luteal phase, iii: fimbria compared to ampulla in the follicular phase. (C) Top gene processes upregulated and downregulated in the fimbria compared to the ampulla as determined by gene ontology. (D) Gene network of top differentially expressed genes by anatomy in the fallopian tube show genes up in the fimbria (green) and ampulla (red). Image was generated using Ingenuity Pathway Analysis software.

A) Gross presentation of the reproductive system in a hen showing the ovary and different parts of the oviduct including oviductal fimbria (FIM), infundibulum (IN), magnum (MG), isthmus (IS), uterus (UT), vagina (VA). B) Microscopic presentation of chicken normal fimbria showing morphological similarities with that of human. C) Gross presentation of fimbrial tumor at early stage accompanied with ascites (ASC). D) Section of a microscopic tumor in fimbria showing papillary-like structure (P). E) Section of a fully formed tumor in fimbria. Ci = Cilia, CL = Cloaca, E = Surface epithelial layer, F₁ to F₅ = Hierarchical follicles, INT = Intestine, L = Lumen, M = Mucosa in fimbria, OV = Ovary, OD = Oviduct, S = Stroma, Tu = Tumor. Scale bar = 20μm.